To unlock the characters below, fulfill the requirement listed.

• Fox Naruto - Beat single player mode with Itachi
• Gaara - Beat the game on the difficult setting
• Guy - Beat the game on any difficulty setting
• Jiraiya - Beat single player mode with Tsunade
• Orichimaru - Beat single player mode with Jiraiya
• Sasuke - Beat arcade mode with each character
• Termari - Beat the game on any difficulty setting
• Tsunade - Beat the game on the difficult setting without losing once